17 May 2011 Fiber-optic confocal microscopy using a miniaturized needle-compatible imaging probe
Author Affiliations +
Proceedings Volume 7753, 21st International Conference on Optical Fiber Sensors; 77534M (2011) https://doi.org/10.1117/12.887373
Event: 21st International Conference on Optical Fibre Sensors (OFS21), 2011, Ottawa, Canada
Abstract
We report on the design and implementation of a 350 μm-diameter confocal imaging probe based on gradient-index (GRIN) optics and a fiber-based scanning arrangement. The form factor of the probe is such that it can potentially be inserted into a 22-gauge hypodermic needle to perform high-resolution confocal fluorescence imaging in solid tissues. We introduce a simple scanning arrangement based on lensed fiber, which eliminates off-axis aberrations induced by conventional scanning optics and is suitable for integration into a compact hand-held unit. We present the details of the optical design and experimental verification of the performance of the optical system. The measured lateral resolution of ~700 nm is in agreement with the optical design and is the highest resolution reported for a confocal fluorescence imaging probe of this size. Further, we demonstrate the imaging capability of the probe by obtaining high-resolution images of fluorescently labeled muscle fibers.
© (2011) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Rajesh S. Pillai, Dirk Lorenser, David D. Sampson, "Fiber-optic confocal microscopy using a miniaturized needle-compatible imaging probe", Proc. SPIE 7753, 21st International Conference on Optical Fiber Sensors, 77534M (17 May 2011); doi: 10.1117/12.887373; https://doi.org/10.1117/12.887373
PROCEEDINGS
4 PAGES


SHARE
Back to Top