Light scattering measurements for quantifying biological cell concentration: an optimization of opto-geometric parameters

A. G. Mignani; L. Ciaccheri; A. A. Mencaglia; L. Giannelli

doi:10.1117/12.884814

17 May 2011 Light scattering measurements for quantifying biological cell concentration: an optimization of opto-geometric parameters

A. G. Mignani, L. Ciaccheri, A. A. Mencaglia, L. Giannelli

Author Affiliations +

Proceedings Volume 7753, 21st International Conference on Optical Fiber Sensors; 77536A (2011) https://doi.org/10.1117/12.884814
Event: 21st International Conference on Optical Fibre Sensors (OFS21), 2011, Ottawa, Canada

Abstract

An experimental study was carried out, aimed at optimizing the optical/geometrical configuration for measuring the concentration of biological cells by means of static light scattering measurements. A LED-based optoelectronic setup making use of optical fibers was experimented, as the precursor of a low-cost device to be integrated in instrumentation for cytometry. Two biological sample types were considered as test samples of the most popular analyses - cervical cells and urine, respectively. The most suitable wavelengths and detecting angles were identified, and calibration curves were calculated.

Citation Download Citation

A. G. Mignani, L. Ciaccheri, A. A. Mencaglia, and L. Giannelli "Light scattering measurements for quantifying biological cell concentration: an optimization of opto-geometric parameters", Proc. SPIE 7753, 21st International Conference on Optical Fiber Sensors, 77536A (17 May 2011); https://doi.org/10.1117/12.884814

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