24 August 2010 Rapid detection of cancer related DNA nanoparticulate biomarkers and nanoparticles in whole blood
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Abstract
The ability to rapidly detect cell free circulating (cfc) DNA, cfc-RNA, exosomes and other nanoparticulate disease biomarkers as well as drug delivery nanoparticles directly in blood is a major challenge for nanomedicine. We now show that microarray and new high voltage dielectrophoretic (DEP) devices can be used to rapidly isolate and detect cfc-DNA nanoparticulates and nanoparticles directly from whole blood and other high conductance samples (plasma, serum, urine, etc.). At DEP frequencies of 5kHz-10kHz both fluorescent-stained high molecular weight (hmw) DNA, cfc-DNA and fluorescent nanoparticles separate from the blood and become highly concentrated at specific DEP highfield regions over the microelectrodes, while blood cells move to the DEP low field-regions. The blood cells can then be removed by a simple fluidic wash while the DNA and nanoparticles remain highly concentrated. The hmw-DNA could be detected at a level of <260ng/ml and the nanoparticles at <9.5 x 109 particles/ml, detection levels that are well within the range for viable clinical diagnostics and drug nanoparticle monitoring. Disease specific cfc-DNA materials could also be detected directly in blood from patients with Chronic Lymphocytic Leukemia (CLL) and confirmed by PCR genotyping analysis.
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Michael J. Heller, Michael J. Heller, Raj Krishnan, Raj Krishnan, Avery Sonnenberg, Avery Sonnenberg, } "Rapid detection of cancer related DNA nanoparticulate biomarkers and nanoparticles in whole blood", Proc. SPIE 7759, Biosensing III, 77590P (24 August 2010); doi: 10.1117/12.861579; https://doi.org/10.1117/12.861579
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