Translator Disclaimer
27 August 2010 DNA tug of war: tether forces on DNA in a nanopore
Author Affiliations +
Optical tweezers are well-known tools for the mechanical manipulation of single molecules in aqueous solutions. Here I will discuss a new development - the combination of optical tweezers with solid-state nanopores. Nanopores are holes in thin membranes usually a few 10s of nm in diameter or even with single nm diameters. In aqueous solutions an ionic current can be driven through a nanopore and thus the translocation of a single molecule detected. Although this information can be used to characterize the length and charge of the molecules, there is no information about the force or position during this process. I will discuss how optical tweezers can be used to mechanically control the translocation process, what we learned so far and where we are going with the technology. In particular, I will show that the optical tweezers/nanopore combination proved to be of exceptional value in unraveling the coupling between electrokinetic and hydrodynamic effects during voltage-driven translocation. This has implication for a wide range of applications ranging from gel electrophoresis to DNA manipulation for lab-on-the chip technology.
© (2010) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Ulrich F. Keyser "DNA tug of war: tether forces on DNA in a nanopore", Proc. SPIE 7762, Optical Trapping and Optical Micromanipulation VII, 776204 (27 August 2010);

Back to Top