27 August 2010 Active laser tweezers microrheometry of microbial biofilms
Author Affiliations +
Microbial biofilms are present on biotic and abiotic surfaces and have a significant impact on many fields in industry, health care and technology. Thus, a better understanding of processes that lead to development of biofilms and their chemical and mechanical properties is needed. In the following paper we report the results of active laser tweezers microrheology study of optically inhomogeneous extracellular matrix secreted by Visbrio sp. bacteria. One particle and two particle active microrheology were used in experiments. Both methods exhibited high enough sensitivity to detect viscosity changes at early stages of bacterial growth. We also showed that both methods can be used in mature samples where optical inhomogeneity becomes significant.
© (2010) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
N. Osterman, V. Slapar, M. Boric, D. Stopar, D. Babič, I. Poberaj, "Active laser tweezers microrheometry of microbial biofilms", Proc. SPIE 7762, Optical Trapping and Optical Micromanipulation VII, 77621P (27 August 2010); doi: 10.1117/12.860423; https://doi.org/10.1117/12.860423


Simulation of active Brownian particles in optical potentials
Proceedings of SPIE (September 16 2014)
Dynamical stabilisation in optical tweezers
Proceedings of SPIE (March 10 2015)
Optical Kapitza pendulum
Proceedings of SPIE (September 16 2016)
Polymeric optical fiber tweezers as a tool for single cell...
Proceedings of SPIE (September 28 2015)

Back to Top