Measurement of macrophage adhesion using optical tweezers with backward-scattered detection

Sung-Yang Wei; Yi-Jr Su; Po-Chen Shih; Shih-Mo Yang; Long Hsu

doi:10.1117/12.860357

27 August 2010 Measurement of macrophage adhesion using optical tweezers with backward-scattered detection

Sung-Yang Wei, Yi-Jr Su, Po-Chen Shih, Shih-Mo Yang, Long Hsu

Proceedings Volume 7762, Optical Trapping and Optical Micromanipulation VII; 77622M (2010) https://doi.org/10.1117/12.860357
Event: SPIE NanoScience + Engineering, 2010, San Diego, California, United States

Abstract

Macrophages are members of the leukocyte family. Tissue damage causes inflammation and release of vasoactive and chemotactic factors, which trigger a local increase in blood flow and capillary permeability. Then, leukocytes accumulate quickly to the infection site. The leukocyte extravasation process takes place according to a sequence of events that involve tethering, activation by a chemoattractant stimulus, adhesion by integrin binding, and migrating to the infection site. The leukocyte extravasation process reveals that adhesion is an important part of the immune system. Optical tweezers have become a useful tool with broad applications in biology and physics. In force measurement, the trapped bead as a probe usually uses a polystyrene bead of 1 μm diameter to measure adhesive force between the trapped beads and cell by optical tweezers. In this paper, using the ray-optics model calculated trapping stiffness and defined the linear displacement ranges. By the theoretical values of stiffness and linear displacement ranges, this study attempted to obtain a proper trapped particle size in measuring adhesive force. Finally, this work investigates real-time adhesion force measurements between human macrophages and trapped beads coated with lipopolysaccharides using optical tweezers with backscattered detection.

Citation Download Citation

Sung-Yang Wei, Yi-Jr Su, Po-Chen Shih, Shih-Mo Yang, and Long Hsu "Measurement of macrophage adhesion using optical tweezers with backward-scattered detection", Proc. SPIE 7762, Optical Trapping and Optical Micromanipulation VII, 77622M (27 August 2010); https://doi.org/10.1117/12.860357

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
7 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Optical tweezers

Adhesives

Geometrical optics

Particles

Beam splitters

Objectives

Blood circulation

Show All Keywords

Keywords/Phrases

Search In:

Publication Years