Haemoglobin-Triton X-100 conjugate as model system for red blood cell lysis

Simona-Florentina Pop; Rodica-Mariana Ion; Sanda Doncea

doi:10.1117/12.882150

3 December 2010 Haemoglobin-Triton X-100 conjugate as model system for red blood cell lysis

Simona-Florentina Pop, Rodica-Mariana Ion, Sanda Doncea

Proceedings Volume 7821, Advanced Topics in Optoelectronics, Microelectronics, and Nanotechnologies V; 78210L (2010) https://doi.org/10.1117/12.882150
Event: Advanced Topics in Optoelectronics, Microelectronics, and Nanotechnologies, 2010, Constanta, Romania

Abstract

The action of detergents is thought to be connected primarily with micelle formation. However, detergent monomers can also affect biological systems. It was found that human red blood cells can be disintegrated with Triton X-100 non-ionic detergent at a concentration of 0.007%, lower than the critical micellar concentration (CMC). The lytic membrane of non-ionic detergent Triton X-100 (as a model), and its ability to lyse red blood cells in vitro used as an indicator of conjugate conformation at different pHs. The time dependent release of hemoglobin (Hb) and potassium from red blood cells was detected at 37 °C and both were sigmoid in character. Although Triton X-100 was highly lytic at pH 5.5, 7.4 and 8.0, the conjugate only show a lysis concentration-dependent of red blood cell at pH 5.5. Triton X-100 causes the Hb to aggregate, a condition that can be simulated when this non-ionic surfactant is incubated with Hb in vitro. The determination of Triton-X was done by HPLC, in accordance to characterize the surfactant. The increased stability in micellar medium can be attributed to deep penetration with the polar group -OH oriented towarded to the micelle surface. Thermal stability of hemoglobin has been investigated in order to evaluate the nature of thermal behavior of this compound. We studied the effects of surfactant Triton -X on the rate constants for the destroying of hemoglobin.

Citation Download Citation

Simona-Florentina Pop, Rodica-Mariana Ion, and Sanda Doncea "Haemoglobin-Triton X-100 conjugate as model system for red blood cell lysis", Proc. SPIE 7821, Advanced Topics in Optoelectronics, Microelectronics, and Nanotechnologies V, 78210L (3 December 2010); https://doi.org/10.1117/12.882150

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available