9 April 2011 New preparation based on coproporphyrin III for photoluminescence diagnostics and photodynamic therapy
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Proceedings Volume 7822, Laser Optics 2010; 78220W (2011) https://doi.org/10.1117/12.896108
Event: Laser Optics 2010, 2010, St. Petersburg, Russian Federation
Abstract
We resume a complex study of a new Coproporphyrin III preparation obtained in vitro via microbiological synthesis in environment of a cultural medium of Arthrobacter globiformis with gaining of the coproporphyrin III tetrapotash salt sterilized aqueous solution with 94.5% purity. The main impurities have porphyrinic nature. The absorption spectrum shows that the preparation has multiple excitation bands in visible region with most pronounced maxima at 501 nm, 535 nm, and 556 nm, and a weak one at 606 nm. The most effective spectral range of its electronic states excitation can serve the region of a Soret band with maximum at 404 nm. Comparative luminescent and photosensitizing properties of Coproporphyrin III and Fotoditazin preparations have been studied. Luminescence and singlet oxygen quantum yields have been determined to be 0.03 and 0.37 (Coproporphyrin III) and 0.05 and 0.6 (Fotoditazin) correspondingly. A reduction of the quantum yields values at an introduction of a biological additive into aqueous solutions of the given preparations has been found. Preclinical trials of the preparation are performed. It is shown that Coproporphyrin III toxicity value is LD50 = 2400 ± 120 mg/kg, that allows to rank this preparation to the (V) class of almost nontoxical medicinal agents. It is shown that the preparation Coproporphyrin III has no teratogenic and allergenic properties and does not damage erythrocytes and thrombocytes. Absence of an influence of the preparation on the blood flow speed has been established. The results obtained thereby testify the absence of contra-indications for clinical tests of the Coproporphyrin III preparation on indicators of sharp toxicity.
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I. M. Belousova, I. M. Belousova, M. V. Dobrun, M. V. Dobrun, L. V. Galebskaya, L. V. Galebskaya, S. I. Gorelov, S. I. Gorelov, I. M. Kislyakov, I. M. Kislyakov, S. E. Kolbasov, S. E. Kolbasov, A. V. Kris'ko, A. V. Kris'ko, T. K. Kris'ko, T. K. Kris'ko, M. A. Malkov, M. A. Malkov, T. D. Murav'eva, T. D. Murav'eva, N. N. Petrishchev, N. N. Petrishchev, } "New preparation based on coproporphyrin III for photoluminescence diagnostics and photodynamic therapy", Proc. SPIE 7822, Laser Optics 2010, 78220W (9 April 2011); doi: 10.1117/12.896108; https://doi.org/10.1117/12.896108
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