8 November 2010 Quantitative analysis of dehydration in porcine skin caused by optical clearing agents
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Dehydration is supposed to be one of mechanisms of optical clearing, but current studies merely gave some qualitative descriptions. Here an analysis method was established to evaluate the water content of skin with PLS method based on the measurements of near-infrared reflectance spectroscopy and weight of porcine skin. Furthermore, a commercial spectrometer with integrating sphere was used to measure the reflectance and transmittance after treatment with different agents. Then the established method was used to evaluate the water content, while the Inverse Adding-Double algorithm was used to calculate the reduced scattering coefficients. The results show that both the water contents and reduced scattering coefficients decrease during the optical clearing process, and there is direct relationship between the optical clearing efficacy and dehydration. With the treating time last, the relative change in reduced scattering coefficient is larger than that in dehydration of skin, and the difference between the changes depends on the agents. Therefore, we conclude that dehydration is the main mechanism of skin optical clearing during the 60 min treatment of the agents, but for some OCAs, i.e., PEG400, glycerol, or D-sorbitol, there might be some other mechanisms contributing to the optical clearing efficacy.
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Tingting Yu, Tingting Yu, Xiang Wen, Xiang Wen, Shu Duan, Shu Duan, Dan Zhu, Dan Zhu, } "Quantitative analysis of dehydration in porcine skin caused by optical clearing agents", Proc. SPIE 7845, Optics in Health Care and Biomedical Optics IV, 78450L (8 November 2010); doi: 10.1117/12.871755; https://doi.org/10.1117/12.871755


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