8 November 2010 Feasibility study for rapid determination of alanine aminotransferase concentration in whole blood by using visible and near infrared spectroscopy
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Abstract
Visible and Near infrared spectroscopy was applied for the fast determination of alanine aminotransferase with whole blood. First, spectra of different thickness (0.5mm, 1mm, 2mm, 4mm ) were investigated to explore Optimal Optical Path for determination. The results show that the whole blood sample with 0.5mm thickness is more suitable for spectral analysis. And then Near infrared spectroscopy of 176 samples were collected. Multiplicative scatter correction and second-order differential method have been used to spectral pretreatment. Stepwise multiple linear regression method and partial least squares regression method have been employed to establish quantitative detection model to predict content of alanine aminotransferase in whole blood. The alanine aminotransferase measured presents best result in calibration and prediction by Near-Infrared Spectroscopy with partial least squares regression calibration model, and the calibration correlation coefficient, the standard error of calibration and the standard error of prediction are 0.98, 2.42 and 7.22 respectively.
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Furong Huang, Zhe Chen, Jianhui Yu, Shiping Li, Yunhan Luo, Shifu Zheng, "Feasibility study for rapid determination of alanine aminotransferase concentration in whole blood by using visible and near infrared spectroscopy", Proc. SPIE 7845, Optics in Health Care and Biomedical Optics IV, 78450O (8 November 2010); doi: 10.1117/12.870251; https://doi.org/10.1117/12.870251
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