8 November 2010 Kinetic analysis of singlet oxygen generation in a living cell using Singlet Oxygen Sensor Green
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Singlet oxygen (1O2) can be generated in a living cell upon focused laser irradiation of an intracellular photosensitizer. In this study, 1O2 generation from the plasma membrane-targeted protoporphyrin IX (PpIX) in human nasopharyngeal carcinoma CNE2 cells was monitored indirectly by using the fluorescence probe Singlet Oxygen Sensor Green agent (SOSG). The confocal images indicate that the fluorescence of SOSG in the vicinity of the cells that incubated with PpIX was dramatically enhanced with the increased irradiation time, while there is no significant enhancement for the control cells. Moreover, the fluorescence of SOSG is dramatically enhanced with the increase of the intracellular PpIX in CNE2 cells for the same photoirradiation time. These observations imply that the 1O2 generated from the plasma membrane-targeted PpIX in the CNE2 cells can be escaped into the extracellular medium and to react with the SOSG to produce SOSG-EP, and the fluorescence enhancement of SOSG around the cells mainly depends on the intracellular PpIX. Our findings may be useful for further monitored the 1O2 that can be escaping from the living cells.
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Yi Shen, Yi Shen, Huiyun Lin, Huiyun Lin, Zufang Huang, Zufang Huang, Lifu Xiao, Lifu Xiao, Defu Chen, Defu Chen, Buhong Li, Buhong Li, Shusen Xie, Shusen Xie, } "Kinetic analysis of singlet oxygen generation in a living cell using Singlet Oxygen Sensor Green", Proc. SPIE 7845, Optics in Health Care and Biomedical Optics IV, 78451F (8 November 2010); doi: 10.1117/12.870261; https://doi.org/10.1117/12.870261

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