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8 November 2010 Laser-induced hard tissue ablation by assisted with a liquid film on target tissue surface
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The objective of this study was to investigate the influence of an applied water film on bone hard tissue ablation by pulse CO2 laser. Fresh bovine shank bone in vitro used in the experiment were put on a PC-controlled motorized linear drive stage and moved repeatedly through focused beam of laser without and with a water film of 0.4 mm and 1 mm on target tissue. The wavelength of pulse CO2 laser was 10.64 μm, pulse repetition rate was 60 Hz, the energy density was 18-84 J/cm2 and the beam diameter of about 400 μm. The moving speed of stage was 12 mm/s, scanning times was 5. The surface morphology and microstructure of ablation grooves were examined by stereomicroscope and scanning electron microscope (SEM) respectively. The geometry of the groove was measured with optical coherence tomography (OCT), and the thermal injury was examined by histology. It shows that water film on the target tissue surface plays an important role during the ablation process. A proper thickness of water film applied to target tissue surface could improve the regular of cut shape, smooth the cut surface, produce the same or even larger ablation rate and efficiency, and reduce the thermal injury around the groove by compared with dry ablation condition. Moreover, the addition of water could alter the microstructure of bone sample.
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Xianzeng Zhang, Haibin Zhao, Zhenlin Zhan, Qing Ye, and Shusen Xie "Laser-induced hard tissue ablation by assisted with a liquid film on target tissue surface", Proc. SPIE 7845, Optics in Health Care and Biomedical Optics IV, 78451N (8 November 2010);

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