8 November 2010 Surfaced-enhanced Raman spectroscopy of nasopharyngeal carcinoma cell using gol nanoparticles
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The surface-enhanced Raman scattering (SERS) spectroscopy and normal Raman spectroscopy of single living human nasopharyngeal carcinoma cells(CNE-1) were tested and analyzed by gold nanoparticles incubation into cells. The characteristic Raman bands in the SERS spectra of living cells were tentatively assigned. Six obvious Raman bands (718, 1001, 1123, 1336, 1446, 1660 cm-1) were observed in the normal Raman spectroscopy of living CNE-1 cells. Colloidal gold nanoparticles that were introduced inside cells resulted in strongly enhanced Raman signals of the native chemical constituents of the cells, and over twenty SERS Raman bands were observed in the SERS spectroscopy of living CNE-1 cells. Four vibrations of the DNA backbone (1026, 1097, 1336 and 1585 cm-1) confirmed that some gold nanoparticles were able to enter the nucleus. The results showed that, based on colloidal gold, the SERS spectroscopy might provide a sensitive and structurally selective detecting method for native chemicals inside a cell, such as DNA and phenylalanine.
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Rong Chen, Hao Huang, Li-qing Sun, Jian-ji Pan, Wei-wei Chen, Ying Su, Shang-yuan Feng, and Yong-zeng Li "Surfaced-enhanced Raman spectroscopy of nasopharyngeal carcinoma cell using gol nanoparticles", Proc. SPIE 7845, Optics in Health Care and Biomedical Optics IV, 784524 (8 November 2010); doi: 10.1117/12.869049; https://doi.org/10.1117/12.869049

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