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8 November 2010 Autofluorescence characteristics of human leukemia cells and mononuclear cells
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Abstract
Autofluorescence spectroscopy is a powerful method to identify the endogenous fluorophores in normal and cancerous cells. The purpose of this study is to characterize the autofluorescence spectra of human normal and leukemia cells. Autofluorescence measurements of each cell line are performed over a wide range of cell concentrations. All of the leukemia cells indicate a statistically significant increase in the tryptophan fluorescence relative to that of the normal cells, while no statistically significant differences are observed in the reduced nicotinamide adenine dinucleotide (phosphate) (NAD(P)H) and flavin adenine dinucleotide (FAD) fluorescence between the normal and leukemia cells. The results suggest that the differences in autofluorescence spectra for leukemia cells and mononuclear cells may be attributed in part to differences in endogenous fluorophores of different cells.
© (2010) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Lifu Xiao, Xiaoying Liao, Lisheng Lin, Huifang Huang, Yuanzhong Chen, and Buhong Li "Autofluorescence characteristics of human leukemia cells and mononuclear cells", Proc. SPIE 7845, Optics in Health Care and Biomedical Optics IV, 78452G (8 November 2010); https://doi.org/10.1117/12.870282
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