Autofluorescence characteristics of human leukemia cells and mononuclear cells

Lifu Xiao; Xiaoying Liao; Lisheng Lin; Huifang Huang; Yuanzhong Chen; Buhong Li

doi:10.1117/12.870282

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8 November 2010 Autofluorescence characteristics of human leukemia cells and mononuclear cells

Lifu Xiao, Xiaoying Liao, Lisheng Lin, Huifang Huang, Yuanzhong Chen, Buhong Li

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Proceedings Volume 7845, Optics in Health Care and Biomedical Optics IV; 78452G (2010) https://doi.org/10.1117/12.870282
Event: Photonics Asia 2010, 2010, Beijing, China

Abstract

Autofluorescence spectroscopy is a powerful method to identify the endogenous fluorophores in normal and cancerous cells. The purpose of this study is to characterize the autofluorescence spectra of human normal and leukemia cells. Autofluorescence measurements of each cell line are performed over a wide range of cell concentrations. All of the leukemia cells indicate a statistically significant increase in the tryptophan fluorescence relative to that of the normal cells, while no statistically significant differences are observed in the reduced nicotinamide adenine dinucleotide (phosphate) (NAD(P)H) and flavin adenine dinucleotide (FAD) fluorescence between the normal and leukemia cells. The results suggest that the differences in autofluorescence spectra for leukemia cells and mononuclear cells may be attributed in part to differences in endogenous fluorophores of different cells.

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Lifu Xiao, Xiaoying Liao, Lisheng Lin, Huifang Huang, Yuanzhong Chen, and Buhong Li "Autofluorescence characteristics of human leukemia cells and mononuclear cells", Proc. SPIE 7845, Optics in Health Care and Biomedical Optics IV, 78452G (8 November 2010); https://doi.org/10.1117/12.870282

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