Research on special type vertical illumination system of metalloscope

Ran Xiao; Ze-xin Xiao; Jie Cao

doi:10.1117/12.869368

9 November 2010 Research on special type vertical illumination system of metalloscope

Ran Xiao, Ze-xin Xiao, Jie Cao

Proceedings Volume 7849, Optical Design and Testing IV; 78491N (2010) https://doi.org/10.1117/12.869368
Event: Photonics Asia 2010, 2010, Beijing, China

Abstract

Kohler illumination is the typical concentrated illumination system of microscope, and is widely used in all kinds of microscopes. Because the conjugate distance of large NA(numerical aperture) kohler illumination system is long, the application of it in general metalloscope is restricted. With the purpose of coordinating the contradiction between them, a kind of large NA and special type vertical kohler illumination is explored according to the optical principle of kohler illumination. Practice has proved that the system can get the same good performance with traditional kohler illumination system. However, the optical tube length and the number of lens of the system are about half of that of the typical structure, and they have higher performance-cost ratio. This paper also introduced a creation design of focal distance offset amount of the annular lens light group using OSLO optical software, and it can be used for dark light group critical lighting through the method of calculating annular lens. The light group has fairly good adaptability with optical properties of the objective, providing a maneuverability practical method of designing refractive dark field critical illumination.

Citation Download Citation

Ran Xiao, Ze-xin Xiao, and Jie Cao "Research on special type vertical illumination system of metalloscope", Proc. SPIE 7849, Optical Design and Testing IV, 78491N (9 November 2010); https://doi.org/10.1117/12.869368

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