11 November 2010 Fluorescence rejection by shifted excitation Raman difference spectroscopy
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Raman spectroscopy is often hampered by strong fluorescence background that can easily bury the much weaker Raman signal. One of the most widely used techniques to reject the fluorescence disturbance is the shifted-excitation Raman difference spectroscopy (SERDS), which incorporates multiple wavelengths as excitation sources. This paper proposed a SERDS system with 532 nm and 526.5 nm DPSS lasers and a home-made holographic notch filter. In this system, two lasers illuminate the sample alternatively, and two series of spectra are acquired to generate the difference spectrum. And then a regularization constrained least square algorithm is proposed to reconstruct the conventional Raman spectrum from the difference spectrum. Computer simulation of this algorithm is provided and the preliminary experimental results are presented. It demonstrates that this system can effectively reject the fluorescence.
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Wenlong Zou, Wenlong Zou, Zhijian Cai, Zhijian Cai, Jianhong Wu, Jianhong Wu, } "Fluorescence rejection by shifted excitation Raman difference spectroscopy", Proc. SPIE 7855, Optical Metrology and Inspection for Industrial Applications, 78551M (11 November 2010); doi: 10.1117/12.869893; https://doi.org/10.1117/12.869893

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