17 February 2011 Monitoring human melanocytic cell responses to piperine using multispectral imaging
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Abstract
Vitiligo is a depigmentary disease characterized by melanocyte loss attributed most commonly to autoimmune mechanisms. Currently vitiligo has a high incidence (1% worldwide) but a poor set of treatment options. Piperine, a compound found in black pepper, is a potential treatment for the depigmentary skin disease vitiligo, due to its ability to stimulate mouse epidermal melanocyte proliferation in vitro and in vivo. The present study investigates the use of multispectral imaging and an image processing technique based on local contrast to quantify the stimulatory effects of piperine on human melanocyte proliferation in reconstructed epidermis. We demonstrate the ability of the imaging method to quantify increased pigmentation in response to piperine treatment. The quantization of melanocyte stimulation by the proposed imaging technique illustrates the potential use of this technology to quickly assess therapeutic responses of vitiligo tissue culture models to treatment non-invasively.
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Ravikant Samatham, Ravikant Samatham, Kevin G. Phillips, Kevin G. Phillips, Julia Sonka, Julia Sonka, Aznegashe Yelma, Aznegashe Yelma, Neha Reddy, Neha Reddy, Meenakshi Vanka, Meenakshi Vanka, Philippe Thuillier, Philippe Thuillier, Amala Soumyanath, Amala Soumyanath, Steven Jacques, Steven Jacques, } "Monitoring human melanocytic cell responses to piperine using multispectral imaging", Proc. SPIE 7883, Photonic Therapeutics and Diagnostics VII, 788309 (17 February 2011); doi: 10.1117/12.875620; https://doi.org/10.1117/12.875620
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