Correlation of spatial intensity distribution of light reaching the retina and restoration of vision by optogenetic stimulation

Shivaranjani Shivalingaiah; Ling Gu; Samarendra K. Mohanty

doi:10.1117/12.875891

11 February 2011 Correlation of spatial intensity distribution of light reaching the retina and restoration of vision by optogenetic stimulation

Shivaranjani Shivalingaiah, Ling Gu, Samarendra K. Mohanty

Author Affiliations +

Proceedings Volume 7885, Ophthalmic Technologies XXI; 78851Y (2011) https://doi.org/10.1117/12.875891
Event: SPIE BiOS, 2011, San Francisco, California, United States

Abstract

Stimulation of retinal neuronal cells using optogenetics via use of chanelrhodopsin-2 (ChR2) and blue light has opened up a new direction for restoration of vision with respect to treatment of Retinitis pigmentosa (RP). In addition to delivery of ChR2 to specific retinal layer using genetic engineering, threshold level of blue light needs to be delivered onto the retina for generating action potential and successful behavioral outcome. We report measurement of intensity distribution of light reaching the retina of Retinitis pigmentosa (RP) mouse models and compared those results with theoretical simulations of light propagation in eye. The parameters for the stimulating source positioning in front of eye was determined for optimal light delivery to the retina. In contrast to earlier viral method based delivery of ChR2 onto retinal ganglion cells, in-vivo electroporation method was employed for retina-transfection of RP mice. The behavioral improvement in mice with Thy1-ChR2-YFP transfected retina, expressing ChR2 in retinal ganglion cells, was found to correlate with stimulation intensity.

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Shivaranjani Shivalingaiah, Ling Gu, and Samarendra K. Mohanty "Correlation of spatial intensity distribution of light reaching the retina and restoration of vision by optogenetic stimulation", Proc. SPIE 7885, Ophthalmic Technologies XXI, 78851Y (11 February 2011); https://doi.org/10.1117/12.875891

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