Effects of 810 nm laser on mouse primary cortical neurons

Gitika B. Kharkwal; Sulbha K. Sharma; Ying-Ying Huang; Luis De Taboada; Thomas McCarthy; Michael R. Hamblin

doi:10.1117/12.876664

17 February 2011 Effects of 810 nm laser on mouse primary cortical neurons

Gitika B. Kharkwal, Sulbha K. Sharma, Ying-Ying Huang, Luis De Taboada, Thomas McCarthy, Michael R. Hamblin

Proceedings Volume 7887, Mechanisms for Low-Light Therapy VI; 788707 (2011) https://doi.org/10.1117/12.876664
Event: SPIE BiOS, 2011, San Francisco, California, United States

Abstract

In the past four decades numerous studies have reported the efficacy of low level light (laser) therapy (LLLT) as a treatment for diverse diseases and injuries. Recent studies have shown that LLLT can biomodulate processes in the central nervous system and has been extensively studied as a stroke treatment. However there is still a lack of knowledge on the effects of LLLT at the cellular level in neurons. The present study aimed to study the effect of 810 nm laser on several cellular processes in primary cortical neurons cultured from mouse embryonic brains. Neurons were irradiated with light dose of 0.03, 0.3, 3, 10 and 30 J/cm² and intracellular levels of reactive oxygen species, nitric oxide and calcium were measured. The changes in mitochondrial function in response to light were studied in terms of adenosine triphosphate (ATP) and mitochondrial membrane potential (MMP). Light induced a significant increase in calcium, ATP and MMP at lower fluences and a decrease at higher fluence. ROS was induced significantly by light at all light doses. Nitric oxide levels also showed an increase on treatment with light. The results of the present study suggest that LLLT at lower fluences is capable of inducing mediators of cell signaling process which in turn may be responsible for the biomodulatory effects of the low level laser. At higher fluences beneficial mediators are reduced but potentially harmful mediators are increased thus offering an explanation for the biphasic dose response.

Citation Download Citation

Gitika B. Kharkwal, Sulbha K. Sharma, Ying-Ying Huang, Luis De Taboada, Thomas McCarthy, and Michael R. Hamblin "Effects of 810 nm laser on mouse primary cortical neurons", Proc. SPIE 7887, Mechanisms for Low-Light Therapy VI, 788707 (17 February 2011); https://doi.org/10.1117/12.876664

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