We aimed assess the effects of different energy densities of the association of red/IR laser light on the healing
of cutaneous wounds infected Staphylococcus aureus. Background: Wound infection is the most common
complication on healing wounds and cause both vascular and cellular responses on the tissue. Several therapeutics is
used for improving wound healing including the use of different light sources, such as the Laser. Some energy
densities present positive photobiological effects on the healing process. Material and Methods: 24 young adult
male Wistar rats, under general anesthesia, had their dorsum shaven, cleaned and a 1 x 1cm cutaneous wound created
with a scalpel and left without no suturing or dressings. The wounds were infected with Staphylococcus aureus and
were randomly divided in 8 subgroups of 3 animals in each: Control, Group 10J/cm2, Group 20J/cm2, and Group
30J/cm2, 7 and 14 days each group. Laser phototherapy was carried out with a diode (λ680nm/790nm, P=
30mW/40mW, CW, Laser, Ø = 3mm, PD=424mW/cm2 and 566mW/cm2, t=11.8/ 8.8 sec, E=0.35J) and started
immediately after surgery and repeated at every other day during 7 days. Laser light was applied on 4 points around
wounded area. The animals were killed at either 8th or 15th day after contamination. Specimens were taken, routinely
cut and processed to wax, stained and underwent histological analysis. The results were statistically analyzed.
Results: Both 20 and 30J/cm2 caused intense collagen deposition at the end of the experimental time. But, when 20
J/cm2 was used the fibers were also well organized. Conclusion: Our results indicate that irradiated subjects showed
improved wound healing being the 20 J/cm2 the energy the caused better histological response.