8 February 2011 Molecular detection via hybrid peptide-semiconductor photonic devices
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Abstract
The aim of this work was to investigate the possibilities to support device functionality that includes strongly confined and localized light emission and detection processes within nano/micro-structured semiconductors for biosensing applications. The interface between biological molecules and semiconductor surfaces, yet still under-explored is a key issue for improving biomolecular recognition in devices. We report on the use of adhesion peptides, elaborated via combinatorial phage-display libraries for controlled placement of biomolecules, leading to user-tailored hybrid photonic systems for molecular detection. An M13 bacteriophage library has been used to screen 1010 different peptides against various semiconductors to finally isolate specific peptides presenting a high binding capacity for the target surfaces. When used to functionalize porous silicon microcavities (PSiM) and GaAs/AlGaAs photonic crystals, we observe the formation of extremely thin (<1nm) peptide layers, hereby preserving the nanostructuration of the crystals. This is important to assure the photonic response of these tiny structures when they are functionalized by a biotinylated peptide layer and then used to capture streptavidin. Molecular detection was monitored via both linear and nonlinear optical measurements. Our linear reflectance spectra demonstrate an enhanced detection resolution via PSiM devices, when functionalized with the Si-specific peptide. Molecular capture at even lower concentrations (femtomols) is possible via the second harmonic generation of GaAs/AlGaAs photonic crystals when functionalized with GaAs-specific peptides. Our work demonstrates the outstanding value of adhesion peptides as interface linkers between semiconductors and biological molecules. They assure an enhanced molecular detection via both linear and nonlinear answers of photonic crystals.
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E. Estephan, E. Estephan, M.-b. Saab, M.-b. Saab, M. Martin, M. Martin, T. Cloitre, T. Cloitre, C. Larroque, C. Larroque, F. J. G. Cuisinier, F. J. G. Cuisinier, A. M. Malvezzi, A. M. Malvezzi, C. Gergely, C. Gergely, } "Molecular detection via hybrid peptide-semiconductor photonic devices", Proc. SPIE 7888, Frontiers in Biological Detection: From Nanosensors to Systems III, 788807 (8 February 2011); doi: 10.1117/12.874691; https://doi.org/10.1117/12.874691
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