CONFERENCE PROCEEDINGS
Advanced Search
Home > Proceedings > Volume 7889 > Article
Open Access Paper
15 February 2011 Magnetomotive optical coherence microscopy for cell dynamics and biomechanics
Xing Liang, Benedikt W. Graf, Renu John, Vasilica Crecea, Freddy T Nguyen, Huafeng Ding, Hyon-min Song, Gabriel Popescu, Alexander Wei, Stephen A. Boppart M.D.
Author Affiliations +
Proceedings Volume 7889, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XV; 788926 (2011) https://doi.org/10.1117/12.875139
Event: SPIE BiOS, 2011, San Francisco, California, United States
Abstract
Magnetomotive microscopy techniques are introduced to investigate cell dynamics and biomechanics. These techniques are based on magnetomotive transducers present in cells and optical coherence imaging techniques. In this study, magnetomotive transducers include magnetic nanoparticles (MNPs) and fluorescently labeled magnetic microspheres, while the optical coherence imaging techniques include integrated optical coherence (OCM)and multiphoton (MPM) microscopy,and diffraction phase microscopy (DPM). Samples used in this study are murine macrophage cells in culture that were incubated with magnetomotive transducers. MPMis used to visualize multifunctional microspheres based on their fluorescence, while magnetomotive OCM detects sinusoidal displacements of the sample induced by a magnetic field. DPM is used to image single cells at a lower frequency magnetic excitation, and with its Fourier transform light scattering (FTLS) analysis, oscillation amplitude is obtained, indicating the relative biomechanical properties of macrophage cells. These magnetomotive microscopy method shave potential to be used to image and measure cell dynamics and biomechanical properties. The ability to measure and understand biomechanical properties of cells and their microenvironments, especially for tumor cells, is of great importance and may provide insight for diagnostic and subsequently therapeutic interventions.
© (2011) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Citation Download Citation
Xing Liang, Benedikt W. Graf, Renu John, Vasilica Crecea, Freddy T Nguyen, Huafeng Ding, Hyon-min Song, Gabriel Popescu, Alexander Wei, and Stephen A. Boppart M.D. "Magnetomotive optical coherence microscopy for cell dynamics and biomechanics", Proc. SPIE 7889, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XV, 788926 (15 February 2011); https://doi.org/10.1117/12.875139
PROCEEDINGS
 7 PAGES
DOWNLOAD PAPER SAVE TO MY LIBRARY
GET CITATION
Lens.org Logo
CITATIONS
Cited by 2 scholarly publications.
Advertisement
Advertisement
RIGHTS & PERMISSIONS
Get copyright permission  Get copyright permission on Copyright Marketplace
KEYWORDS
Magnetism
Modulation
Microscopy
Transducers
Nanoparticles
Light scattering
Diffraction
RELATED CONTENT
Subscribe to Digital Library
Receive Erratum Email Alert
Back to Top