22 February 2011 Spectrum resolved fluorescence imaging in multi-focal volume holographic microscopy
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Abstract
A real-time three dimensional (3D) fluorescence imaging system incorporating wavelength-coded and multiplexed holographic gratings is presented. Holographic gratings formed in thick Phenanthrenquinone- (PQ-) Doped Poly (methyl methacrylate) (PMMA) have narrowband spectral-spatial transmittance filtering properties to generate wavelengthspectrum selective multi-focal planes within a biological object. We demonstrate the imaging modality to obtain laserinduced fluorescent tissue structures from different depths at the excitation wavelength of 355nm.
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Yuan Luo, Yuan Luo, Ioannis Zervantonakis, Ioannis Zervantonakis, Se Baek Oh, Se Baek Oh, Roger Kamm, Roger Kamm, George Barbastathis, George Barbastathis, } "Spectrum resolved fluorescence imaging in multi-focal volume holographic microscopy", Proc. SPIE 7891, Design and Quality for Biomedical Technologies IV, 78910C (22 February 2011); doi: 10.1117/12.874570; https://doi.org/10.1117/12.874570
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