22 February 2011 Spectrum resolved fluorescence imaging in multi-focal volume holographic microscopy
Author Affiliations +
A real-time three dimensional (3D) fluorescence imaging system incorporating wavelength-coded and multiplexed holographic gratings is presented. Holographic gratings formed in thick Phenanthrenquinone- (PQ-) Doped Poly (methyl methacrylate) (PMMA) have narrowband spectral-spatial transmittance filtering properties to generate wavelengthspectrum selective multi-focal planes within a biological object. We demonstrate the imaging modality to obtain laserinduced fluorescent tissue structures from different depths at the excitation wavelength of 355nm.
© (2011) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Yuan Luo, Yuan Luo, Ioannis Zervantonakis, Ioannis Zervantonakis, Se Baek Oh, Se Baek Oh, Roger Kamm, Roger Kamm, George Barbastathis, George Barbastathis, "Spectrum resolved fluorescence imaging in multi-focal volume holographic microscopy", Proc. SPIE 7891, Design and Quality for Biomedical Technologies IV, 78910C (22 February 2011); doi: 10.1117/12.874570; https://doi.org/10.1117/12.874570

Back to Top