Different optical spectral characteristics in a necrotic transmissible venereal tumor and a cystic lesion in the same canine prostate observed by triple-band trans-rectal optical tomography under trans-rectal ultrasound guidance

Zhen Jiang; G. Reed Holyoak; Jerry W. Ritchey; Kenneth E. Bartels; Kendra Rock; Charlotte L. Ownby; Gennady Slobodov; Charles F. Bunting; Daqing Piao

doi:10.1117/12.873144

10 February 2011 Different optical spectral characteristics in a necrotic transmissible venereal tumor and a cystic lesion in the same canine prostate observed by triple-band trans-rectal optical tomography under trans-rectal ultrasound guidance

Zhen Jiang, G. Reed Holyoak, Jerry W. Ritchey, Kenneth E. Bartels, Kendra Rock, Charlotte L. Ownby, Gennady Slobodov, Charles F. Bunting, Daqing Piao

Author Affiliations +

Proceedings Volume 7892, Multimodal Biomedical Imaging VI; 78920P (2011) https://doi.org/10.1117/12.873144
Event: SPIE BiOS, 2011, San Francisco, California, United States

Abstract

Different optical spectral characteristics were observed in a necrotic transmissible venereal tumor (TVT) and a cystic lesion in the same canine prostate by triple-wavelength trans-rectal optical tomography under trans-rectal ultrasound (TRUS) guidance. The NIR imager acquiring at 705nm, 785nm and 808nm was used to quantify both the total hemoglobin concentration (HbT) and oxygen saturation (StO2) in the prostate. The TVT tumor in the canine prostate as a model of prostate cancer was induced in a 7-year old, 27 kg dog. A 2 mL suspension of 2.5x10⁶ cells/mL of homogenized TVT cells recovered from an in vivo subcutaneously propagated TVT tumor in an NOD/SCID mouse were injected in the cranial aspect of the right lobe of the canine prostate. The left lobe of the prostate had a cystic lesion present before TVT inoculation. After the TVT homogenate injection, the prostate was monitored weekly over a 9-week period, using trans-rectal NIR and TRUS in grey-scale and Doppler. A TVT mass within the right lobe developed a necrotic center during the later stages of this study, as the mass presented with substantially increased [HbT] in the periphery, with an area of reduced StO2 less than the area of the mass itself shown on ultrasonography. Conversely, the cystic lesion presented with slightly increased [HbT] in the periphery of the lesion shown on ultrasound with oxygen-reduction inside and in the periphery of the lesion. There was no detectable change of blood flow on Doppler US in the periphery of the cystic lesion. The slightly increased [HbT] in the periphery of the cystic lesion was correlated with intra-lesional hemorrhage upon histopathologic examination.

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Zhen Jiang, G. Reed Holyoak, Jerry W. Ritchey, Kenneth E. Bartels, Kendra Rock, Charlotte L. Ownby, Gennady Slobodov, Charles F. Bunting, and Daqing Piao "Different optical spectral characteristics in a necrotic transmissible venereal tumor and a cystic lesion in the same canine prostate observed by triple-band trans-rectal optical tomography under trans-rectal ultrasound guidance", Proc. SPIE 7892, Multimodal Biomedical Imaging VI, 78920P (10 February 2011); https://doi.org/10.1117/12.873144

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