Development of a hybrid MRI and fluorescence tomography system for small animal imaging

Michael T. Ghijsen; Yuting Lin; Orhan Nalcioglu; Gultekin Gulsen

doi:10.1117/12.881166

21 February 2011 Development of a hybrid MRI and fluorescence tomography system for small animal imaging

Michael T. Ghijsen, Yuting Lin, Orhan Nalcioglu, Gultekin Gulsen

Proceedings Volume 7892, Multimodal Biomedical Imaging VI; 789212 (2011) https://doi.org/10.1117/12.881166
Event: SPIE BiOS, 2011, San Francisco, California, United States

Abstract

Fluorescence diffuse optical tomography (FT) is a molecular imaging technique that can create images of spatially resolved fluorophore concentrations and fluorescence lifetimes. One problem faced by FT is that the recovered fluorophore parameters greatly depend on the size and depth of the inclusion due to the ill-posedness of the FT inverse problem. Structural a priori information from imaging modalities with high spatial resolution is demonstrated to significantly improve the accuracy of the FT reconstruction. We have constructed a hybrid FT/MRI system for small animal imaging in this study. Near-infrared light was delivered and collected by optical fibers that connect the FT/MRI system to the interface in the MRI bore. We investigated the feasibility of a photo-multiplier tube (PMT) based detection system that acquired time-resolved data in the frequency domain. Phantom studies were used to evaluate the performance of the combined system. The concentration and lifetime maps were reconstructed with and without the structural a priori information obtained from MRI. ICG and DTTCI, two fluorophores with similar excitation and emission spectra but different lifetimes, were used in this evaluation. Specifically, we showed that the PMT-based frequency domain hybrid system was capable of differentiating between two fluorophores with different fluorescence lifetimes. Furthermore, this process was shown to be more accurate when MR a priori is used.

Citation Download Citation

Michael T. Ghijsen, Yuting Lin, Orhan Nalcioglu, and Gultekin Gulsen "Development of a hybrid MRI and fluorescence tomography system for small animal imaging", Proc. SPIE 7892, Multimodal Biomedical Imaging VI, 789212 (21 February 2011); https://doi.org/10.1117/12.881166

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available