22 February 2011 Toward nonlinear endomicroscopy for exploration of the pulmonary airways: preliminary spectroscopic study of human lung tissue using a single-mode fibre
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Proceedings Volume 7893, Endoscopic Microscopy VI; 789309 (2011) https://doi.org/10.1117/12.875440
Event: SPIE BiOS, 2011, San Francisco, California, United States
Abstract
We are developing a non-linear fibered endomicroscope for imaging the extracellular matrix collagen and elastin fibrillar networks during bronchoscopy. As a proof of concept, laser pulses at the output of a standard 2 meter long single-mode fibre have been obtained with pulse duration of about 50 fs and pulse energy up to 50 uJ, using a specially designed grism line for the dispersion compensation. With these pulses, we performed a spectroscopic characterization of the non-linear endogenous signal, consisting of two-photon fluorescence and second harmonic generation, and originated from human pulmonary tissue of various thickness, both in forward and backward geometry of signal collection, with excitation at 830 nm.
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Donald A. Peyrot, Donald A. Peyrot, Claire Lefort, Claire Lefort, D. Abi Haidar, D. Abi Haidar, Tigran Mansuryan, Tigran Mansuryan, Sergeï Kruglik, Sergeï Kruglik, Nicolas Sandeau, Nicolas Sandeau, Frédéric Louradour, Frédéric Louradour, Geneviève Bourg-Heckly, Geneviève Bourg-Heckly, } "Toward nonlinear endomicroscopy for exploration of the pulmonary airways: preliminary spectroscopic study of human lung tissue using a single-mode fibre", Proc. SPIE 7893, Endoscopic Microscopy VI, 789309 (22 February 2011); doi: 10.1117/12.875440; https://doi.org/10.1117/12.875440
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