Toward nonlinear endomicroscopy for exploration of the pulmonary airways: preliminary spectroscopic study of human lung tissue using a single-mode fibre

Donald A. Peyrot; Claire Lefort; D. Abi Haidar; Tigran Mansuryan; Sergeï Kruglik; Nicolas Sandeau; Frédéric Louradour; Geneviève Bourg-Heckly

doi:10.1117/12.875440

22 February 2011 Toward nonlinear endomicroscopy for exploration of the pulmonary airways: preliminary spectroscopic study of human lung tissue using a single-mode fibre

Donald A. Peyrot, Claire Lefort, D. Abi Haidar, Tigran Mansuryan, Sergeï Kruglik, Nicolas Sandeau, Frédéric Louradour, Geneviève Bourg-Heckly

Author Affiliations +

Proceedings Volume 7893, Endoscopic Microscopy VI; 789309 (2011) https://doi.org/10.1117/12.875440
Event: SPIE BiOS, 2011, San Francisco, California, United States

Abstract

We are developing a non-linear fibered endomicroscope for imaging the extracellular matrix collagen and elastin fibrillar networks during bronchoscopy. As a proof of concept, laser pulses at the output of a standard 2 meter long single-mode fibre have been obtained with pulse duration of about 50 fs and pulse energy up to 50 uJ, using a specially designed grism line for the dispersion compensation. With these pulses, we performed a spectroscopic characterization of the non-linear endogenous signal, consisting of two-photon fluorescence and second harmonic generation, and originated from human pulmonary tissue of various thickness, both in forward and backward geometry of signal collection, with excitation at 830 nm.

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Donald A. Peyrot, Claire Lefort, D. Abi Haidar, Tigran Mansuryan, Sergeï Kruglik, Nicolas Sandeau, Frédéric Louradour, and Geneviève Bourg-Heckly "Toward nonlinear endomicroscopy for exploration of the pulmonary airways: preliminary spectroscopic study of human lung tissue using a single-mode fibre", Proc. SPIE 7893, Endoscopic Microscopy VI, 789309 (22 February 2011); https://doi.org/10.1117/12.875440

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