Ex-vivo ultra-high-resolution optical coherence tomography imaging of fine lung structure by use of a high-power Gaussian-like supercontinuum at 0.8-um wavelength

N. Nishizawa; S. Ishida; T. Ohta; K. Itoh; M. Kitatsuji; H. Ohshima; Y. Hasegawa; M. Matsushima; T. Kawabe

doi:10.1117/12.878874

22 February 2011 Ex-vivo ultra-high-resolution optical coherence tomography imaging of fine lung structure by use of a high-power Gaussian-like supercontinuum at 0.8-μm wavelength

N. Nishizawa, S. Ishida, T. Ohta, K. Itoh, M. Kitatsuji, H. Ohshima, Y. Hasegawa, M. Matsushima, T. Kawabe

Author Affiliations +

Proceedings Volume 7893, Endoscopic Microscopy VI; 78930Y (2011) https://doi.org/10.1117/12.878874
Event: SPIE BiOS, 2011, San Francisco, California, United States

Abstract

Optical coherence tomography (OCT) is an emerging technology for non-invasive cross-sectional imaging of biological tissue and material with um resolution. Recently, non-invasive high resolution cross-sectional imaging is desired for investigation of diseases in lung in the field of pulmonary medicine. So far, a few works have been reported about OCT imaging of lung. Since the lung consists of alveoli separated by thin wall, ultrahigh resolution (UHR) OCT is supposed to be effective for the imaging of fine structure in lung tissue. In this work, ex vivo cross-sectional imaging of isolated rat lungs was demonstrated using UHR-OCT. A 120 nm-wide, high-power, Gaussian-like supercontinuum (SC) was generated at wavelength of 0.8 um region and it was used as the light source in time domain UHR-OCT. An ultrahigh resolution of 2.1 um in tissue was obtained and the achieved sensitivity was 105 dB. For the UHR-OCT imaging of trachea, the detailed structures of the tracheal cartilage and tracheal mucosa overlying the cartilage were observed clearly. The epithelium and lamina propria were also distinguishable. For the imaging of visceral pleura and alveoli, when saline was instilled into the lung, the penetration depth was improved, and clear images of the fine structure of the lung, including alveoli, were observed owing to the index matching effect. The clear images of up to about 4 alveoli were observed below the visceral pleura. The shape of the alveolar septum was clearly observed, and the alveolar sac was clearly visible.

Citation Download Citation

N. Nishizawa, S. Ishida, T. Ohta, K. Itoh, M. Kitatsuji, H. Ohshima, Y. Hasegawa, M. Matsushima, and T. Kawabe "Ex-vivo ultra-high-resolution optical coherence tomography imaging of fine lung structure by use of a high-power Gaussian-like supercontinuum at 0.8-μm wavelength", Proc. SPIE 7893, Endoscopic Microscopy VI, 78930Y (22 February 2011); https://doi.org/10.1117/12.878874

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available