Multiphoton imaging and quantification of tissue glycation

Ara A. Ghazaryan; Jo-Ya Tseng; Wen Lo; Yang-Fang Chen; Vladimir Hovhannisyan; Shean-Jen Chen; Hsin-Yuan Tan; Chen-Yuan Dong

doi:10.1117/12.874536

16 February 2011 Multiphoton imaging and quantification of tissue glycation

Ara A. Ghazaryan, Jo-Ya Tseng, Wen Lo, Yang-Fang Chen, Vladimir Hovhannisyan, Shean-Jen Chen, Hsin-Yuan Tan, Chen-Yuan Dong

Author Affiliations +

Proceedings Volume 7895, Optical Biopsy IX; 789509 (2011) https://doi.org/10.1117/12.874536
Event: SPIE BiOS, 2011, San Francisco, California, United States

Abstract

In this work, we applied multi-photon microscopy to image and characterize the extent of tissue glycation. Specifically, features of multi-photon autofluorescence (MPAF) and second harmonic generation (SHG) images were presented. Our study shows that multiphoton imaging is capable of providing qualitative and quantitative information of the extent of tissue glycation in ribose treated samples including bovine sclera and vessel and that this approach may be useful for clinical evaluation of advanced glycation endproducts (AGEs) formation.

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Ara A. Ghazaryan, Jo-Ya Tseng, Wen Lo, Yang-Fang Chen, Vladimir Hovhannisyan, Shean-Jen Chen, Hsin-Yuan Tan, and Chen-Yuan Dong "Multiphoton imaging and quantification of tissue glycation", Proc. SPIE 7895, Optical Biopsy IX, 789509 (16 February 2011); https://doi.org/10.1117/12.874536

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