Detection of cervical cancer by fluorescence emission and stokes' shift spectra of blood and urine

V. Masilamani; T. Vijmasi; M. AlSalhi; K. Govindarajan; A. P. VijayaRaghavan; Ram Rathan Rai

doi:10.1117/12.874010

16 February 2011 Detection of cervical cancer by fluorescence emission and stokes' shift spectra of blood and urine

V. Masilamani, T. Vijmasi, M. AlSalhi, K. Govindarajan, A. P. VijayaRaghavan, Ram Rathan Rai

Proceedings Volume 7895, Optical Biopsy IX; 78950A (2011) https://doi.org/10.1117/12.874010
Event: SPIE BiOS, 2011, San Francisco, California, United States

Abstract

In this paper we present the results of a study to distinguish cervical cancer patients [ N=50] from healthy subjects [N=50] based on the Fluorescence Emission Spectra [FES] and Stokes' Shift Spectra [SSS] of blood and urine. FES was obtained from the cellular fraction of blood and urine by excitation at 400 nm. SSS was obtained from blood plasma and urine with Δλ of 70nm. In the FES of blood cellular fraction, the ratio of intensity of the two bands due to neutral porphyrin and basic porphyrin [I630 / I580] was 1 for normal controls and 3 for cervical cancers. In the SSS of plasma, the average ratio of intensity of the two bands due to tryptophan and collagen [I305 nm / I340 nm] was 1.9 for normal controls, 1.1 for early cervical cancers and 0.9 for advanced cervical cancers In the SSS of urine, the ratio of intensity of the two bands due to flavin and NADH [I450 nm / I360 nm] was 0.2 for normal controls and 0.8 for cancer patients. A discriminant analysis combining all three parameters showed a sensitivity of 80% and specificity of 78% for this technique. In this study we show that fluorescence spectroscopy of blood and urine could develop into a promising technique for non-invasive diagnosis and screening of cervical cancers and would appropriately supplement or complement currently used techniques.

Citation Download Citation

V. Masilamani, T. Vijmasi, M. AlSalhi, K. Govindarajan, A. P. VijayaRaghavan, and Ram Rathan Rai "Detection of cervical cancer by fluorescence emission and stokes' shift spectra of blood and urine", Proc. SPIE 7895, Optical Biopsy IX, 78950A (16 February 2011); https://doi.org/10.1117/12.874010

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