Radiofrequency circuit design and performance evaluation for small animal frequency-domain NIR fluorescence optical tomography

Chinmay Darne; Banghe Zhu; Yujie Lu; I-Chih Tan; John Rasmussen; Eva M. Sevick-Muraca

doi:10.1117/12.874946

17 February 2011 Radiofrequency circuit design and performance evaluation for small animal frequency-domain NIR fluorescence optical tomography

Chinmay Darne, Banghe Zhu, Yujie Lu, I-Chih Tan, John Rasmussen, Eva M. Sevick-Muraca

Author Affiliations +

Proceedings Volume 7896, Optical Tomography and Spectroscopy of Tissue IX; 789621 (2011) https://doi.org/10.1117/12.874946
Event: SPIE BiOS, 2011, San Francisco, California, United States

Abstract

Herein we report on hardware development and evaluation for frequency-domain photon migration (FDPM) technique that is miniaturized for incorporation into a micro-CT gantry for hybrid CT/NIR/PET imaging. Immunity to endogenous optical properties and enhanced contrast associated with fluorophore lifetime is inherent to the FDPM measurements and enables unique opportunities for quantitative tomography when compared to the time independent (continuous wave) approach. A miniaturized radiofrequency (rf) circuitry has been developed in our laboratory for homodyne FDPM measurements that makes use of a single 100MHz oscillator to simultaneously launch optically modulated excitation light into a small animal as well as to modulate an NIR sensitive image intensifier for collection of fluorescent signals. The use of a single oscillator not only eliminates signal drift that otherwise results from the use of multiple oscillators individually driving both source and detector, but also reduces the circuit footprint for incorporation into the CT gantry. Herein, overall system performance parameters of signal-to-noise ratio, measurement precision, spatial resolution, modulation depth (ac/dc), excitation light rejection, and clinically relevant data acquisition times are presented for mouse phantom data. Image reconstruction of phantom data and integration of circuitry for hybrid CT/NIR/PET imaging is also presented towards the ultimate validation of NIR optical tomography using PET imaging as a "gold-standard" for quantification.

Citation Download Citation

Chinmay Darne, Banghe Zhu, Yujie Lu, I-Chih Tan, John Rasmussen, and Eva M. Sevick-Muraca "Radiofrequency circuit design and performance evaluation for small animal frequency-domain NIR fluorescence optical tomography", Proc. SPIE 7896, Optical Tomography and Spectroscopy of Tissue IX, 789621 (17 February 2011); https://doi.org/10.1117/12.874946

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