Multisite and multidepth tumors localization enhancement after autofluorescence removal

Anne-Sophie Montcuquet; Lionel Hervé; Fabrice Navarro; Jean-Marc Dinten; Jérôme I. Mars

doi:10.1117/12.874594

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17 February 2011 Multi-site and multi-depth in vivo cancer localization enhancement after autofluorescence removal

Anne-Sophie Montcuquet; Lionel Hervé; Fabrice Navarro; Jean-Marc Dinten; Jérôme I. Mars

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Proceedings Volume 7896, Optical Tomography and Spectroscopy of Tissue IX; 78962S (2011) https://doi.org/10.1117/12.874594
Event: SPIE BiOS, 2011, San Francisco, California, United States

Abstract

Fluorescence imaging in diffusive media locates tumors tagged by injected fluorescent markers in NIR wave-lengths. For deep embedded markers, natural autofluorescence of tissues comes to be a limiting factor to tumor detection and accurate FDOT reconstructions. A spectroscopic approach coupled with Non-negative Matrix Factorization source separation method is explored to discriminate fluorescence sources according to their fluorescence spectra and remove unwanted autofluorescence. We successfully removed autofluorescence from acquisitions on living mice with a single subcutaneous tumor or two capillary tubes inserted at different depths.

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Anne-Sophie Montcuquet, Lionel Hervé, Fabrice Navarro, Jean-Marc Dinten, and Jérôme I. Mars "Multi-site and multi-depth in vivo cancer localization enhancement after autofluorescence removal", Proc. SPIE 7896, Optical Tomography and Spectroscopy of Tissue IX, 78962S (17 February 2011); https://doi.org/10.1117/12.874594

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