17 February 2011 Laser induced detachment and re-orientation of cells
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Abstract
Re-orientation of adhering cell(s) with respect to other cell(s) has not been yet possible, thus limiting study of controlled interaction between cells. Here, we report cell detachment upon irradiation with a focused near-infrared laser beam, and reorientation of adherent cells. The cell gets detached after irradiation for few seconds, followed by vertical orientation. The detached cell was transported along axial direction by scattering force and trapped at a higher plane inside the media using the same laser beam by Gravito-optical trap. The trapped cell could then be repositioned by movement of the sample stage and reoriented by rotation of the astigmatic trapping beam. The height at which the cell was stably held was found to depend on the laser beam power. The cell could be brought back to the substrate by reducing the laser beam power using a polarizer or blocking the laser beam. Viability of the detached and manipulated cell was found not to be compromised as confirmed by PI fluorescence exclusion assay. The re-oriented cell was allowed to re-attach to the substrate at a controlled distance and orientation with respect to other cells. Further, the cell was found to retain its shape even after multiple detachments and manipulation using the laser beam. This technique opens up new avenues for non-contact modification of cellular orientations that will enable study of inter-cellular interactions and design of engineered tissue.
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Ling Gu, Ling Gu, Ninad Ingle, Ninad Ingle, Samarendra K. Mohanty, Samarendra K. Mohanty, } "Laser induced detachment and re-orientation of cells", Proc. SPIE 7897, Optical Interactions with Tissue and Cells XXII, 78970B (17 February 2011); doi: 10.1117/12.875952; https://doi.org/10.1117/12.875952
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