17 February 2011 Characterizing temperature-dependent photo-oxidation to explain the abrupt transition from thermal to non-thermal laser damage mechanisms at 413 nm
Author Affiliations +
Abstract
Laser exposure duration dictates whether tissues subjected to short visible wavelengths ( ≤ 514 nm) are damaged by thermal (e.g. 0.1 s) or non-thermal ( ≥ 100 s) mechanisms. Somewhere between these extremes, an abrupt transition between the two damage mechanisms has been found for both in vitro and animal retinal models (J. Biomed. Opt. 15, 030512, 2010). Non-thermal (photochemical) damage is characterized by an inverse relationship between damage threshold irradiance and exposure duration (irradiance reciprocity). We have found that exposures of 40 - 60 s in an in vitro retinal model require radiant exposures well above the expected requirement for nonthermal damage, introducing the concept that damage was forced to be thermal in mechanism. Here we quantify and compare photo-oxidative processes at ambient temperatures between 35 - 50 °C.
© (2011) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Michael L. Denton, C. D. Clark, Gary D. Noojin, Larry E. Estlack, Adam C. Schenk, Curtis W. Burney, Benjamin A. Rockwell, and Robert J. Thomas "Characterizing temperature-dependent photo-oxidation to explain the abrupt transition from thermal to non-thermal laser damage mechanisms at 413 nm", Proc. SPIE 7897, Optical Interactions with Tissue and Cells XXII, 78970K (17 February 2011); doi: 10.1117/12.873469; https://doi.org/10.1117/12.873469
PROCEEDINGS
7 PAGES


SHARE
Advertisement
Advertisement
RELATED CONTENT

Effects of temperature on fluorescence in human tissue
Proceedings of SPIE (February 23 2010)
An in-vitro model for retinal laser damage
Proceedings of SPIE (February 20 2007)

Back to Top