17 February 2011 Three-dimensional angular domain optical projection tomography
Author Affiliations +
Abstract
Angular Domain Imaging (ADI) has been previously demonstrated to generate projection images of attenuating targets embedded within a turbid medium. The imaging system employs a silicon micro-tunnel array positioned between the sample and the detection system to reject scattered photons that have deviated from the initial propagation direction and to select for ballistic and quasi-ballistic photons that have retained their forward trajectory. Two dimensional tomographic images can be reconstructed from ADI projections collected at a multitude of angles. The objective of this work was to extend the system to three dimensions by collecting several tomographic images and stacking the reconstructed slices to generate a three dimensional volume representative of the imaging target. A diode laser (808nm, CW) with a beam expander was used to illuminate the sample cuvette. An Angular Filter Array (AFA) of 80 μm × 80 μm square-shaped tunnels 2 cm in length was used to select for image forming quasi-ballistic photons. Images were detected with a linear CCD. Our approach was to use a SCARA robot to rotate and translate the sample to collect sufficient projections to reconstruct a three dimensional volume. A custom designed 3D target consisting of 4 truncated cones was imaged and reconstructed with filtered backprojection and iterative methods. A 0.5 mm graphite rod was used to collect the forward model, while a truncated pseudoinverse was used to approximate the backward model for the iterative algorithm.
© (2011) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Eldon Ng, Eldon Ng, Fartash Vasefi, Fartash Vasefi, Michael Roumeliotis, Michael Roumeliotis, Bozena Kaminska, Bozena Kaminska, Jeffrey J. L. Carson, Jeffrey J. L. Carson, } "Three-dimensional angular domain optical projection tomography", Proc. SPIE 7897, Optical Interactions with Tissue and Cells XXII, 78970V (17 February 2011); doi: 10.1117/12.875828; https://doi.org/10.1117/12.875828
PROCEEDINGS
8 PAGES


SHARE
Back to Top