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28 February 2011 Photothermal therapy of acute leukemia cells in the near-infrared region using gold nanorods CD-33 conjugates
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In the present work, we demonstrate a potential use of gold nanorods as a contrast agent for selective photothermal therapy of human acute leukemia cells (HL-60) using a near-infrared laser. Gold Nanorods (GNR) are synthesized and conjugated to CD33, a 67 kDa glycoprotein found on the surface of myeloid cells that belongs to the sialoadhesin family of proteins. After pegylation, or conjugation with CD33 antibody, GNR were non-toxic for acute and chronic leukemia cells. We used a Quanta System q-switched titanium sapphire laser emitting at a center wavelength of 755 nm. Each sample was illuminated with 1 laser shot at either high or low fluence. Both laser modes were used in 3 independent cell probes. HL-60 cells were treated for 45 min with GNR conjugated with mAb CD33, or with GNR-Pegylated particles. After laser application, the cells were resuspended and analyzed to cell viability with Trypan blue exclusion assay. GNR-CD33 conjugates significantly increase the percentage of cell death as compared with a control group after laser illumination: a 3 fold increase is observed.
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Anton Liopo, André Conjusteau, Marina Konopleva, Michael Andreeff, and Alexander Oraevsky "Photothermal therapy of acute leukemia cells in the near-infrared region using gold nanorods CD-33 conjugates", Proc. SPIE 7897, Optical Interactions with Tissue and Cells XXII, 789710 (28 February 2011);


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