Optoacoustic sensor for nanoparticle linked immunosorbent assay (NanoLISA)

André Conjusteau; Anton Liopo; Dmitri Tsyboulski; Sergey A. Ermilov; William R. Elliott III; Norman Barsalou; Saher M. Maswadi; Randolph D. Glickman; Alexander A. Oraevsky

doi:10.1117/12.879401

28 February 2011 Optoacoustic sensor for nanoparticle linked immunosorbent assay (NanoLISA)

André Conjusteau, Anton Liopo, Dmitri Tsyboulski, Sergey A. Ermilov, William R. Elliott III, Norman Barsalou, Saher M. Maswadi, Randolph D. Glickman, Alexander A. Oraevsky

Author Affiliations +

Proceedings Volume 7899, Photons Plus Ultrasound: Imaging and Sensing 2011; 789910 (2011) https://doi.org/10.1117/12.879401
Event: SPIE BiOS, 2011, San Francisco, California, United States

Abstract

We developed an optoacoustic biosensor intended for the detection of bloodborne microorganisms using immunoaffinity reactions of antibody-coupled gold nanorods as contrast agents specifically targeted to the antigen of interest. Optoacoustic responses generated by the samples are detected using a wide band ultrasonic transducer. The sensitivity of the technique has been assessed by determining minimally detectable optical density which corresponds to the minimum detectable concentration of the target viral surface antigens. Both ionic solutions and gold nanorods served as the contrast agent generating the optoacoustic response. The sensitivity of Nano-LISA is at least OD=10-6 which allows reliable detection of 1 pg/ml (depending on the commercial antibodies that are used). Adequate detection sensitivity, as well as lack of non-specific cross-reaction between antigens favors NanoLISA as a viable technology for biosensor development.

Citation Download Citation

André Conjusteau, Anton Liopo, Dmitri Tsyboulski, Sergey A. Ermilov, William R. Elliott III, Norman Barsalou, Saher M. Maswadi, Randolph D. Glickman, and Alexander A. Oraevsky "Optoacoustic sensor for nanoparticle linked immunosorbent assay (NanoLISA)", Proc. SPIE 7899, Photons Plus Ultrasound: Imaging and Sensing 2011, 789910 (28 February 2011); https://doi.org/10.1117/12.879401

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