17 February 2011 Photoacoustic imaging of gene expression using tyrosinase as a reporter gene
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Abstract
Optical reporter genes, such as green fluorescence protein, are powerful research tools that allow visualization of gene expression. We have successfully used tyrosinase as a reporter gene for photoacoustic imaging. Tyrosinase is the key regulatory enzyme in the production of melanin which has a broad optical absorption spectrum. MCF-7 cells were stably transfected with tyrosinase under the control of an inducible promoter. For photoacoustic experiments, MCF-7 cells were resuspended at 108 cells/mL and injected in 700 μm (inner diameter) plastic tubing. Photoacoustic signal of MCF-7 cells expressing tyrosinase were >20-fold greater than those of untransfected MCF-7 cells. Photoacoustic signal of tyrosinaseexpressing MCF-7 cells were approximately 2-fold lesser and greater than those of blood at 576 and 650 nm, respectively, suggesting that photoacoustic signal from blood and tyrosinase-expressing cells can be separated by dualwavelength analysis. Photoacoustic signal from tyrosinase-expressing MCF-7 cells covered by chicken tissue could even be detected at a laser penetration depth of 4 cm, suggesting that tyrosinase can be used to image gene expression in relatively deep tissues. The current data suggests that tyrosinase is a strong reporter gene for photoacoustic imaging.
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Robert J. Paproski, Robert J. Paproski, Alexander Forbrich, Alexander Forbrich, Tyler Harrison, Tyler Harrison, Mary Hitt, Mary Hitt, Roger J. Zemp, Roger J. Zemp, } "Photoacoustic imaging of gene expression using tyrosinase as a reporter gene", Proc. SPIE 7899, Photons Plus Ultrasound: Imaging and Sensing 2011, 78991H (17 February 2011); doi: 10.1117/12.875197; https://doi.org/10.1117/12.875197
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