Dual-mode photoacoustic microscopy of carbon nanotube incorporated scaffolds in blood and biological tissues

Xin Cai; Song Hu; Bhavna Paratala; Balaji Sitharaman; Lihong V. Wang

doi:10.1117/12.873230

18 February 2011 Dual-mode photoacoustic microscopy of carbon nanotube incorporated scaffolds in blood and biological tissues

Xin Cai, Song Hu, Bhavna Paratala, Balaji Sitharaman, Lihong V. Wang

Author Affiliations +

Proceedings Volume 7899, Photons Plus Ultrasound: Imaging and Sensing 2011; 78992I (2011) https://doi.org/10.1117/12.873230
Event: SPIE BiOS, 2011, San Francisco, California, United States

Abstract

Three-dimensional scaffolds provide physical support and an adjustable microenvironment to facilitate vascularization of ischemic tissues; however, in vivo imaging of scaffold functioning is still challenging. Micro-CT, the current frequentlyused imaging modality for scaffold characterization, provides poor contrast for wet scaffold, which limits its in vivo applications. In this paper, dual modes of photoacoustic microscopy (PAM), using acoustic resolution PAM (AR-PAM) and optical resolution PAM (OR-PAM), were employed for imaging scaffolds in blood as well as in chicken breast tissues. By choosing different wavelengths, 570 nm and 638 nm, we spectroscopically differentiated the photoacoustic signals generated from blood and from carbon nanotube incorporated scaffolds. The ex vivo experiments demonstrated a lateral resolution of 45 μm and a maximum penetration of ~2 mm for AR-PAM, and a lateral resolution of 3 μm and a maximum penetration of ~660 μm for OR-PAM. OR-PAM further quantified the average pore size of scaffolds to be 100-200 μm in diameter. Our results suggest that PAM is a promising tool for in vivo monitoring of scaffold-induced angiogenesis as well as the degradability of scaffolds themselves.

Citation Download Citation

Xin Cai, Song Hu, Bhavna Paratala, Balaji Sitharaman, and Lihong V. Wang "Dual-mode photoacoustic microscopy of carbon nanotube incorporated scaffolds in blood and biological tissues", Proc. SPIE 7899, Photons Plus Ultrasound: Imaging and Sensing 2011, 78992I (18 February 2011); https://doi.org/10.1117/12.873230

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available