22 February 2011 Do large fluorescent particles enhance the modulation efficiency of ultrasound-modulated fluorescence?
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Abstract
The question of whether particle size affects modulation efficiency, defined as the ratio of ultrasound-modulated fluorescence (UMF) signal to DC (direct current) signal, of the fluorescence emission from four different sized fluorescent particles was investigated experimentally. The four particles are streptavidin-conjugated Alexa Fluo 647 (~5 nm in diameter) and three carboxylate-modified fluorescent microspheres (FM) with different diameters of 0.02, 0.2, and 1.0 μm. Modulation efficiency was evaluated as a function of the fluorophore size and fluorophore concentration. The modulation efficiency was improved about two times when the size of the fluorescent particles is increased from 5 nm to 1 μm. This result implies that using large fluorescence particles can slightly improve the modulation efficiency but the improvement is limited.
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Yuan Liu, Yuan Liu, Baohong Yuan, Baohong Yuan, Joseph Vignola, Joseph Vignola, "Do large fluorescent particles enhance the modulation efficiency of ultrasound-modulated fluorescence?", Proc. SPIE 7899, Photons Plus Ultrasound: Imaging and Sensing 2011, 789934 (22 February 2011); doi: 10.1117/12.874906; https://doi.org/10.1117/12.874906
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