Total internal reflection photoacoustic detection spectroscopy

Amanda S. M. Sudduth; Benjamin S. Goldschmidt; Edward B. Samson; Paul J. D. Whiteside; John A. Viator

doi:10.1117/12.875313

22 February 2011 Total internal reflection photoacoustic detection spectroscopy

Amanda S. M. Sudduth, Benjamin S. Goldschmidt, Edward B. Samson, Paul J. D. Whiteside, John A. Viator

Proceedings Volume 7899, Photons Plus Ultrasound: Imaging and Sensing 2011; 78993E (2011) https://doi.org/10.1117/12.875313
Event: SPIE BiOS, 2011, San Francisco, California, United States

Abstract

Total Internal Reflection Photoacoustic Spectroscopy (TIRPAS) is a method that exploits the evanescent field of a nanosecond duration laser pulse reflecting off a glass/water interface to generate photoacoustic responses. These photoacoustic events are generated in light absorbing analytes suspended in the fluid medium in contact with the glass that are within the penetration depth of the evanescent wave. This method has been employed in previous studies by Hinoue et al. Hinoue et al. used an optically chopped HeNe laser at 632.8 nm to detect Brilliant Blue FCF dye at different angles of incidence. In recent years, the advent of high power nanosecond pulsed tunable lasers has allowed for the re-visitation of the TIRPAS idea under stress confinement and orders of magnitude larger peak energy conditions. Compared to conventional detection methods, this approach has the potential to detect much smaller quantities of disease indicators, such as circulating tumor cells and hemazoin crystals in malaria, than other optical methods. The detection limit of the TIRPAS system was quantified using chlorazol black solution with an absorption coefficient of 55 cm^-1 at 532 nm. Interaction with the evanescent field was verified by varying the angle of incidence of the probe laser beam that generated the photoacoustic waves, thereby changing the penetration depth of the evanescent field as well as the photoacoustic spectroscopy effect from angled excitation.

Citation Download Citation

Amanda S. M. Sudduth, Benjamin S. Goldschmidt, Edward B. Samson, Paul J. D. Whiteside, and John A. Viator "Total internal reflection photoacoustic detection spectroscopy", Proc. SPIE 7899, Photons Plus Ultrasound: Imaging and Sensing 2011, 78993E (22 February 2011); https://doi.org/10.1117/12.875313

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