Taxol-induced paraptosis-like A549 cell death is not senescence

Chao-yang Wang; Tong-Sheng Chen

doi:10.1117/12.877056

10 February 2011 Taxol-induced paraptosis-like A549 cell death is not senescence

Chao-yang Wang, Tong-Sheng Chen

Proceedings Volume 7900, Biophotonics and Immune Responses VI; 79000K (2011) https://doi.org/10.1117/12.877056
Event: SPIE BiOS, 2011, San Francisco, California, United States

Abstract

Our previous studies have shown that taxol, a potent anticancer agent, induces caspase-independent cell death and cytoplasmic vacuolization in human lung cancer cells. However, the mechanisms of taxol-induced cytoplasmic vacuolization are poorly understood. Cytoplasmic vacuolization have been reported to be a characteristic of cell senescence. Here, we employed confocal fluorescence microscopy imaging to study the reversibility of taxol-induced cytoplasmic vacuolization and whether taxol triggers senescence in A549 cells. We found that taxol-induced cytoplasmic vacuolization at 6 or 9 h after treatment with taxol did not decrease but increase at 24 h or 72 h after refreshing the culture medium without taxol, indicating taxol-induced cytoplasmic vacuolization is irreversible. We used SA-β-Gal (senescence-associated β-galactosidase) to assess whether taxol-induced cell death in cytoplasmic vacuolization fashion is senescence, and found that hydrogen peroxide (H₂O₂)-treated, but not taxol-treated cells is significantly stained by the SA-β-Gal, a senescence testing kit, indicating that the form of taxol-induced cell death is not senescence.

Citation Download Citation

Chao-yang Wang and Tong-Sheng Chen "Taxol-induced paraptosis-like A549 cell death is not senescence", Proc. SPIE 7900, Biophotonics and Immune Responses VI, 79000K (10 February 2011); https://doi.org/10.1117/12.877056

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