10 February 2011 Taxol-induced paraptosis-like A549 cell death is not senescence
Author Affiliations +
Proceedings Volume 7900, Biophotonics and Immune Responses VI; 79000K (2011) https://doi.org/10.1117/12.877056
Event: SPIE BiOS, 2011, San Francisco, California, United States
Abstract
Our previous studies have shown that taxol, a potent anticancer agent, induces caspase-independent cell death and cytoplasmic vacuolization in human lung cancer cells. However, the mechanisms of taxol-induced cytoplasmic vacuolization are poorly understood. Cytoplasmic vacuolization have been reported to be a characteristic of cell senescence. Here, we employed confocal fluorescence microscopy imaging to study the reversibility of taxol-induced cytoplasmic vacuolization and whether taxol triggers senescence in A549 cells. We found that taxol-induced cytoplasmic vacuolization at 6 or 9 h after treatment with taxol did not decrease but increase at 24 h or 72 h after refreshing the culture medium without taxol, indicating taxol-induced cytoplasmic vacuolization is irreversible. We used SA-β-Gal (senescence-associated β-galactosidase) to assess whether taxol-induced cell death in cytoplasmic vacuolization fashion is senescence, and found that hydrogen peroxide (H2O2)-treated, but not taxol-treated cells is significantly stained by the SA-β-Gal, a senescence testing kit, indicating that the form of taxol-induced cell death is not senescence.
© (2011) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Chao-yang Wang, Chao-yang Wang, Tong-Sheng Chen, Tong-Sheng Chen, } "Taxol-induced paraptosis-like A549 cell death is not senescence", Proc. SPIE 7900, Biophotonics and Immune Responses VI, 79000K (10 February 2011); doi: 10.1117/12.877056; https://doi.org/10.1117/12.877056
PROCEEDINGS
6 PAGES


SHARE
Back to Top