22 February 2011 Arrhenius parameters for primary thermal injury in human tonsillar tissue
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Abstract
Clinical implementation of a thermal therapy requires the ability to predict tissue injury following exposures to specific thermal histories. As part of an effort to develop a nonexcisional alternative to tonsillectomy, the degree of primary hyperthermic tissue injury in human tonsil was characterized. Fifteen fresh pediatric hypertrophic tonsillectomy specimens were sectioned and treated in a NIST-calibrated saline bath at temperatures of 40 to 70°C with hold times of one to seven minutes. The treated tissues were subsequently nitroblue tetrazolium (NBT) stained to assess for thermal respiratory enzyme inactivation as a marker of cellular injury/death. The NBT stains were quantitatively image analyzed and used to calculate Arrhenius parameters for primary thermal injury in human tonsils.
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Kathleen McMillan, Rebecca Radabaugh, James E. Coad, "Arrhenius parameters for primary thermal injury in human tonsillar tissue", Proc. SPIE 7901, Energy-based Treatment of Tissue and Assessment VI, 790110 (22 February 2011); doi: 10.1117/12.876338; https://doi.org/10.1117/12.876338
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