10 February 2011 Multispectral line confocal imaging microscope for fluorescence applications
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Abstract
We present a novel line confocal microscope for investigating biological samples that simultaneously acquires images from three fluorescent channels. This instrument provides the majority of the benefits of confocal microscopes, such as depth sectioning and improved contrast, while allowing more of the excitation illumination to reach the sample area, resulting in reduced exposure times. One configuration of the line confocal fluorescent microscope is designed to work with a four laser illuminator, and three discrete cameras, while an alternate configuration allows the instrument to act as a multispectral microscope that captures the fluorescent emission over the visible and NIR wavelength range.
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Mark M. Meyers, Mark M. Meyers, Iza Ferreira, Iza Ferreira, Pavel Fomitchov, Pavel Fomitchov, Robert Filkins, Robert Filkins, } "Multispectral line confocal imaging microscope for fluorescence applications", Proc. SPIE 7902, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX, 79020I (10 February 2011); doi: 10.1117/12.875447; https://doi.org/10.1117/12.875447
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