28 February 2011 Transillumination of subcutaneous adipose tissues using near-infrared hyperspectral imaging in the 1100-1800 nm wavelength range
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Abstract
Hyperspectral imaging (HSI) is a chemical imaging modality with spectroscopic information. This technique was often used in agricultural or pharmaceutical industries. But there have been a few reports for clinical medical applications. In near-infrared (NIR) wavelength region, the significant absorption peaks are often observed by the overtone of midinfrared molecular vibration. In addition, NIR light has a high penetration because of low scattering and less absorption by water or protein. In this study, we constructed the NIR-HSI system and the high-contrast subcutaneous adipose tissue imaging was conducted in-vitro. In the absorption spectra which are obtained by our NIR-HSI system, the characteristic absorption bands were observed around 1200 nm and 1700 nm. In the processed images using these wavelength bands, subcutaneous adipose tissue was observed through a skin. In a hyperspectral image by another processing using all wavelengths, a high-contrast image of subcutaneous adipose tissue is also obtained. NIR-HSI system is a powerful diagnostic technique for adipose tissues distribution and their morphological change on/inside a tissue.
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K. Ishii, K. Ishii, A. Kitayabu, A. Kitayabu, Y. Kobayashi, Y. Kobayashi, N. Honda, N. Honda, K. Awazu, K. Awazu, "Transillumination of subcutaneous adipose tissues using near-infrared hyperspectral imaging in the 1100-1800 nm wavelength range", Proc. SPIE 7902, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX, 79021X (28 February 2011); doi: 10.1117/12.874540; https://doi.org/10.1117/12.874540
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