8 February 2011 Elastic fibers and collagen distribution in human aorta
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Abstract
Elastic and collagen fibers are essential components of the aorta, the remodeling of these structures is accompanied with aging in various diseases and life-threatening events. While the elastic fibers confer resilience to major blood vessels collagen confers resistance to the same. Elastic fibers are easily visualized in the fluorescent light when stained with hematoxylin eosin. Second Harmonic Generation (SHG) is a non linear signal that occurs only in molecules without inversion symmetry and is particularly strong in the collagen fibers arranged in triple helices. The aim of this paper is to describe the distribution of collagen in the thickness of the thoracic aorta, and to demonstrate the distribution of between elastic fibers. The images were acquired in a multifoton microscopy and both signals, Two-phtoton excitaded fluorescence (TPEF) and SHG, were excited by a Ti:Sapphire laser. We used a band pass filter to filter the SHG signal from the TPEF signal. The thickness of the aorta varies 2-3 mm, and the image was composed of the juxtaposition of images of 220 x 220 microns. We acquired images of a histological slide of the thoracic aorta stained with picrosirius red (specific for collagen) at a wavelength of 670nm SHG subsequently acquired images with the same region and observed that the images are overlapping. Therefore, the following images were acquired by confocal microscopy (fluorescence of eosin for visualization of elastic fibers) and for collagen SHG. After reconstruction of the images, we observed the distribution of collagen along the aorta.
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G. Vieira-Damiani, G. Vieira-Damiani, D. P. Ferro, D. P. Ferro, R. L. Adam, R. L. Adam, A. A. de Thomaz, A. A. de Thomaz, V. Pelegati, V. Pelegati, C. L. Cesar, C. L. Cesar, K. Metze, K. Metze, "Elastic fibers and collagen distribution in human aorta", Proc. SPIE 7903, Multiphoton Microscopy in the Biomedical Sciences XI, 79030B (8 February 2011); doi: 10.1117/12.874695; https://doi.org/10.1117/12.874695
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