11 February 2011 Quantitative analysis of diseased horse tendons using Fourier-transform-second-harmonic generation imaging
Author Affiliations +
Fourier transform-second-harmonic generation (FT-SHG) imaging is used to quantitatively assess the structural organization of collagen fibers in tendonitis-induced horse tendons. Fiber orientation, isotropy, and the ratio of forward to backward SHG signal (F/B ratio) are used to differentiate the fiber organization between the normal and diseased horse tendons. Each second-harmonic generation (SHG) image is divided into several smaller regions of interest (ROI) and the aforementioned quantitative metrics are calculated across the whole grid. ROIs are further labeled as dark (no or minimal presence of fibers), isotropic (random fiber organization), or anisotropic (regular fiber organization) regions. Results show that the normal tendon possesses minimal isotropic regions and small standard deviations in the histograms of orientation and F/B ratio, indicating an intact and highly regular fiber organization. However, the tendonitis-induced horse tendons possess higher number of dark and isotropic regions, and larger standard deviations of the measured parameters, suggesting significantly disoriented and disorganized collagen fibers. This type of quantification would be highly beneficial in diagnosing and determining the stage of tendonitis in clinical settings. Not limited to tendonitis, the technique could also be applied to other diseases that structurally affect collagen fibers. The advantage of FT-SHG over the conventional polarization microscopy is also discussed.
© (2011) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Mayandi Sivaguru, Mayandi Sivaguru, Sushmitha Durgam, Sushmitha Durgam, Raghu Ambekar, Raghu Ambekar, David Luedtke, David Luedtke, Glenn Fried, Glenn Fried, Allison Stewart, Allison Stewart, Kimani C. Toussaint, Kimani C. Toussaint, } "Quantitative analysis of diseased horse tendons using Fourier-transform-second-harmonic generation imaging", Proc. SPIE 7903, Multiphoton Microscopy in the Biomedical Sciences XI, 79030J (11 February 2011); doi: 10.1117/12.875586; https://doi.org/10.1117/12.875586

Back to Top