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22 February 2011 High contrast coherent anti-Stokes Raman scattering microscopy using tightly focused cylindrical vector beams
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Abstract
We report on a unique annular aperture detection scheme in radially and linearly polarized coherent anti-Stokes Raman scattering (RP- and LP-CARS) microscopy to effectively remove the solvent background for high contrast vibrational imaging. Our finite-difference time-domain (FDTD) calculations show that the far-field CARS radiation from the scatterer with size comparable to the excitation wavelength is stronger than that from the solvent at large cone angles (from 45° to the maximum collection angle of the objective). The annular detection provides about one to two orders higher contrast for forward detected RP- and LP-CARS microscopy.
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Jian Lin, Fake Lu, Wei Zheng, and Zhiwei Huang "High contrast coherent anti-Stokes Raman scattering microscopy using tightly focused cylindrical vector beams", Proc. SPIE 7903, Multiphoton Microscopy in the Biomedical Sciences XI, 79031F (22 February 2011); https://doi.org/10.1117/12.873674
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